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演講快訊
Protein Activity Preserved by Aqueous Two Phase System (APTS) Consisting of Poly(ethylene glycol) (PEG) and Hyaluronic Acid
[2019/04/25]
教務
Protein Activity Preserved by Aqueous Two Phase System (APTS) Consisting of Poly(ethylene glycol) (PEG) and Hyaluronic Acid

Seminar Date/Time

 Friday, April 25, 2019 at 1.30 pm    hosted by詹正雄 教授

                                                     posted by:陳菊英 助教

Speaker

 Prof. Tooru Ooya

 

Affiliation

Kobe University

 

Seminar Title

 Protein Activity Preserved by Aqueous Two Phase System (APTS) Consisting of Poly(ethylene glycol) (PEG) and Hyaluronic Acid

 

Location

 創意講堂

 

Language

 English

 

Abstract

    Aqueous two phase system (APTS) consisting of poly(ethylene glycol) (PEG) and polysaccharides have been studied for easy purification of proteins and cell particles. ATPS has been focused on not only bio-separation but also controlled release of proteins or PEGylated proteins.  Herein, PEG-grafted hyaluronic acid (PEG-g-HA) and the crosslinked hydrogels were evaluated for controlled protein delivery systems toward diabetes therapy regenerative medicine. Preparative condition of PEG-g-HA was examined in order to easily modulate the graft number of PEG. PEG-g-HA was prepared by a condensation reaction between HA and a mono-amino PEG. The number of PEG was controlled by just feed ratio of PEG and HA.

Correlation between insulin release profile and in vivo activity in terms of blood glucose level was investigated by subcutaneous injection of PEG-g-HA containing insulin to rats.  In vitro insulin release behavior showed in a sustained manner.  This result suggests that the exercise of PEG microdomain contributed to the partition of insulin to the microdomain. In addition, from the results of subcutaneous administration to rats, blood glucose level decreased, and the low level was kept longer than the control samples (insulin, insulin/PEG). These results strongly suggest that the sustained release of insulin from the PEG microdomain was also observed in blood, and the insulin activity was maintained due to the partition of insulin to the PEG-surrounded environment.

       In the field of regenerative medicine, controlled release of basic fibroblast growth factor (bFGF) from scaffolds has been extensively studied to achieve reliable tissue regeneration. The PEG-g-HA system was applied to the controlled release of bFGF and vascularization.  The fluorescein isothiocyanate (FITC)-labeled bFGF loading to HA crosslinked hydrogels and the release behavior were analyzed by fluorescence measurements.  The release of FITC-bFGF from the PEG-g-HA crosslinked hydrogel with PEG grafting decreased the release rate, strongly suggesting that the PEG grafting induced some physical crosslinking.  The bFGF-loaded hydrogels were subcutaneously implanted to mouse for one week, and the results suggests that the bFGF was released in a sustained manner, and the released bFGF induced significant vascularization. 

  

Education/Work Experience

1997 Graduate School of Materials Science (Doctoral Course), Japan Advanced Institute of Science and Technology, Ishikawa, Japan 
1995 Graduate School of Materials Science (Master's Course), Japan Advanced Institute of Science and Technology, Ishikawa, Japan 
1993 Graduated from Food and Nutritional Science, University of Shizuoka, Shizuoka, Japan 
  

2008-   Associate Professor, Graduate School of Engineering, Faculty of Engineering, Kobe University, Kobe, Japan
2006-2008 Associate Professor, Department of Intelligent Systems Design Engineering, and Biotechnology Research Center, Toyama Prefectural University, Toyama, Japan.
2001-2002 Visiting Research Scholar, Department of Industrial and Physical Pharmacy (IPPH), Purdue University, West Lafayette, IN, USA.
1997-2005 Research Assistant Professor, School of Materials Science, Japan Advanced Institute of Science and Technology, Ishikawa, Japan.
  


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