Fabricating biomimetic in vitro tissue/organ-based systems for drug testing is an important topic in tissue engineering field. However, the interactions among cells, materials, and signals are complex so that there are many challenges existing in the fabrication of biomimetic models. In this topic, we will introduce the strategies to develop various in vitro neural cell-based models by use serum components. Through the serum fraction less than 100 kDa (100KD medium), we could obtain a neuron-differentiation medium to create an in vitro neuron-rich model. In addition, we developed a NSPC proliferation medium containing epithelial grow factor and fibronectin (Medium B), which can be used to obtain a NSPC-rich model. Moreover, we will further use the single- and multi-materials bioprinting techniques for making biomimetic 3D constructs. Through these techniques, it could solve the current issue that 2D materials are difficult to provide a complex 3D environment for culturing cells. In the furture, staring from neural tissue engineering, we hope to provide the results to fabricate various neural cell-based models for fabricating biomimetic neural tissue/organ platforms for drug testing.